Dissociation of Antigen-Antibody Complexes for some Plant Viruses in DAS-ELISA Tests

Antigen-Antibody Immune Complexes

Development of an ELISA Test for Detection of Anti FMD Virus Type A87/IRN Antibody Using Irradiated Antigen

Background and Aims: The aim of this study was application of gamma irradiated FMDV antigen for liquid phase blocking sandwich ELISA (B-ELISA) to quantify guinea pig anti FMDV type A87/IRN antibody. Methods: FMDV type A87/IRN was propagated to a titer of 107.5/ml TCID50 and then irradiated by gamma irradiator. The irradiated 146 S antigen was purified by sucrose gradient centrifugation after in...

the washback effect of discretepoint vs. integrative tests on the retention of content in knowledge tests

در این پایان نامه تاثیر دو نوع تست جزیی نگر و کلی نگر بر به یادسپاری محتوا ارزیابی شده که نتایج نشان دهندهکارایی تستهای کلی نگر بیشتر از سایر آزمونها است

Improvement of Leishmania major Amastigote ELISA Test Using Various Antigen Antibody Dissociating Agents and Pepsin Digestion.

Studies on Antigen-antibody Complexes

Solid phase immunoadsorbents were prepared by coupling antigens to agarose. With this technique specific antibodies were easily isolated in large amounts. The gammaG-globulin class of antibodies isolated in this manner were not denatured as judged by their normal biological half-life in rabbits. Soluble immune complexes at fivefold antigen excess were prepared from isolated specific antibodies ...